Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (49): 9232-9235.doi: 10.3969/j.issn.2095-4344.2012.49.020

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Naringin induces bone marrow mesenchymal stem cells to repair femoral head necrosis in rabbits

Yang Yuan1, Li Xiao-feng1, Luo Dao-ming2, Wen Chao-hai2   

  1. 1Guangxi Orthopedic Traumatology Hospital, Nanning 530012, Guangxi Zhuang Autonomous Region, China; 2Guangxi Traditional Chinese Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China
  • Online:2012-12-02 Published:2013-01-16
  • Contact: Li Xiao-feng, Master, Guangxi Orthopedic Traumatology Hospital, Nanning 530012, Guangxi Zhuang Autonomous Region, China lxfeng2000@126.com E-mail: lxfeng2000@126.com
  • Supported by:

    a grant from Department of Science and Technology of Guangxi Zhuang Autonomous Region of China, No. Gui-ke-gong0816004-15*; a grant from Health Department of Guangxi Zhuang Autonomous Region of China, No. Zhong200863*

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Abstract:

BACKGROUND: Autologous bone marrow mesenchymal stem cells exhibit multipotential differentiation after in vitro culture. There is increasing concern that whether transplantation of autologous bone marrow mesenchymal stem cells can be used for repair of femoral head necrosis.
OBJECTIVE: To investigate the mechanism by which naringin induces bone marrow mesenchymal stem cells to repair femoral head necrosis in rabbits. 
METHODS: Rabbit bone marrow mesenchymal stem cells were induced with naringin to differentiate into osteoblasts and then loaded onto cancellous bone allograft. Femoral head necrosis rabbit models were prepared. At 3 weeks after femoral head necrosis induction, 12 adult New Zealand rabbits were randomly divided into two groups. In the experimental group, blank cancellous bone allograft was implanted into the left femoral head necrosis region. Identically, nothing was implanted into the left femoral head necrosis region in the control group. The right femoral head necrosis region in each group was implanted with cancellous bone allograft loaded by naringin induced bone marrow mesenchymal stem cells.
RESULTS AND CONCLUTION: At 8 weeks after implantation, a large amount of callus formed on the surface of the allograft and connected tightly with bone ends, and continuous “bone bridge” formed in some parts. At 12 weeks, relatively mature bone trabeculae were observed in the filling area, which were thicker, and arranged more irregularly compared with normal bone trabeculae. Bone defect region was completely covered by the newly formed bone. These findings suggest that naringin-induced bone marrow mesenchymal stem cells can be used for repair of femoral head necrosis in rabbits.

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